Anticorps Polyclonal de lapin anti-Prion protein PrP/CD230

Prion protein PrP/CD230 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12555-1-PBS

Synonymes

Prion protein PrP, PRNP, PrP, Alternative prion protein, AltPrP

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Galerie de données de validation

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  WB analysis of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PrP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12555-1-AP (same clone as 12555-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PRNP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12555-1-AP (same clone as 12555-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12555-1-AP (PrP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 12555-1-AP (same clone as 12555-1-PBS)

  IP result of anti-PrP (IP:12555-1-AP, 4ug; Detection:12555-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12555-1-AP (same clone as 12555-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12555-1-AP (PrP Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 12555-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12555-1-PBS cible Prion protein PrP/CD230 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Prion protein PrP/CD230 Protéine recombinante Ag3257
• Nom complet: prion protein
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC022532
• Symbole du gène: PrP
• Identification du gène (NCBI): 5621
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prion protein (PRNP) is a ubiquitous membrane glycoprotein whose abnormal self-replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Prion diseases are TSEs, attributed to conformational conversion of the cellular prion protein (PrPC) into an abnormal conformer that accumulates in the brain. The two isoforms, PrPC and PrPS, have the same primary amino acid sequence and only differ in conformation. While PrPC is composed of 42% α-helix and only 3% β-sheet, PrPSc is composed of 30% α-helix and 43% β-sheet. PrPC converts to its pathogenic isoform when the region corresponding to the residues 108-144 fold into β-sheets. PrPC is very soluble in detergents and easily digested by proteases while the PrPSc is insoluble in detergents and resistant to protease digestion. Prion diseases exist in infectious, sporadic, and genetic forms.

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