Anticorps Polyclonal de lapin anti-Proenkephalin-A

Proenkephalin-A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32272-1-PBS

Synonymes

PENK, Proenkephalin A, Opioid growth factor, Met-enkephalin-Arg-Phe, Met-enkephalin-Arg-Gly-Leu

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Galerie de données de validation

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  WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (PENK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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  WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

  Fetal human brain was subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 32272-1-AP (same clone as 32272-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 32272-1-AP (same clone as 32272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 32272-1-AP (same clone as 32272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 32272-1-AP (Proenkephalin-A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 32272-1-AP (same clone as 32272-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Proenkephalin-A antibody (32272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 32272-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32272-1-PBS cible Proenkephalin-A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Proenkephalin-A Protéine recombinante Ag27972
• Nom complet: proenkephalin
• Masse moléculaire calculée: 267 aa, 31 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC032505
• Symbole du gène: Proenkephalin A
• Identification du gène (NCBI): 5179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Proenkephalin-A (PENK) is also named as Synenkephalin, Met-enkephalin and Opioid growth factor (OGF), and belongs to the opioid neuropeptide precursor family. PENK is a neuropeptide that competes with and mimics the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (PMID:7057924). PENK hypermethylation is also associated with bladder cancer, hepatocellular carcinoma, colorectal cancer, and prostate cancer (PMID:36403035). Proenkephalin (PENK) represents a new candidate to determine kidney function. This peptide is cleaved from the precursor peptide preproenkephalin A alongside enkephalins (endogenous opioids) and is filtrated in the glomerulus (PMID: 33636859).

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