Anticorps Monoclonal anti-Prohibitin

Prohibitin Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4D3G5

N° de cat : 60092-1-PBS

Synonymes

PHB, 4D3G5, PHB1, Prohibitin 1

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Galerie de données de validation

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  WB analysis using 60092-1-Ig (same clone as 60092-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60092-1-Ig (Prohibitin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 60092-1-Ig (same clone as 60092-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60092-1-Ig (PHB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60092-1-Ig (Prohibitin Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 60092-1-Ig(PHB antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60092-1-Ig (Prohibitin antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 60092-1-Ig (same clone as 60092-1-PBS)

  Immunofluorescent analysis of Hela cells, using PHB antibody 60092-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 60092-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60092-1-PBS cible Prohibitin dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Prohibitin Protéine recombinante Ag1240
• Nom complet: prohibitin
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC013401
• Symbole du gène: Prohibitin
• Identification du gène (NCBI): 5245
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prohibitin, also known as PHB, a member of the Band-7 family of proteins, is widely distributed in bacteria, plants, yeast, protozoa and mammals and is important in cell proliferation, differentiation and apoptosis (PMID: 20840588 ). This protein localizes to the inner membrane of mitochondria, where it acts as a chaperone protein, and is also found in the nucleus, where it negatively regulates transcription (PMID: 18558096). Recent study confirmed that the expression and distribution of PHB, which is a nuclear matrix protein, affect the apoptosis of HaCaT cells and its co-localization with specific gene products connected with cell apoptosis (PMID: 24402549).

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