Anticorps Recombinant de lapin anti-Prolactin

Prolactin Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241771A5

N° de cat : 85042-1-PBS

Synonymes

241771A5

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Galerie de données de validation

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  WB analysis using 85042-1-RR (same clone as 85042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85042-1-RR (Prolactin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide and mouse spleen tissue slide (negative control) using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85042-1-RR against Mouse Prolactin were performed. The affinity constant is 4.50 nM.

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Informations sur le produit

85042-1-PBS cible Prolactin dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Prolactin Protéine recombinante Eg0937
• Nom complet: prolactin
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NP_035294.2
• Symbole du gène: Prl
• Identification du gène (NCBI): 19109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056).

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