Anticorps Recombinant de lapin anti-Prolactin

Prolactin Recombinant Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

241771A5

N° de cat : 85042-1-RR

Synonymes

241771A5

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Galerie de données de validation

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  WB analysis using 85042-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85042-1-RR (Prolactin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pituitary gland using 85042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pituitary gland using 85042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide and mouse spleen tissue slide (negative control) using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pituitary gland using 85042-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse pituitary gland using 85042-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85042-1-RR against Mouse Prolactin were performed. The affinity constant is 4.50 nM.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu pulmonaire de rat, tissu splénique de rat
• Résultats positifs en IHC: mouse pituitary gland tissue, tissu splénique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: mouse pituitary gland tissue,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85042-1-RR cible Prolactin dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Prolactin Protéine recombinante Eg0937
• Nom complet: prolactin
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: NP_035294.2
• Symbole du gène: Prl
• Identification du gène (NCBI): 19109
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056).

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