Anticorps Monoclonal anti-Prostein

Prostein Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5E12E2

N° de cat : 60343-1-PBS

Synonymes

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

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Galerie de données de validation

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  WB analysis using 60343-1-Ig (same clone as 60343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 60343-1-Ig (same clone as 60343-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 60343-1-Ig (same clone as 60343-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60343-1-PBS cible Prostein dans les applications de WB, IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Prostein Protéine recombinante Ag5463
• Nom complet: solute carrier family 45, member 3
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 55-59 kDa
• Numéro d’acquisition GenBank: BC050416
• Symbole du gène: Prostein
• Identification du gène (NCBI): 85414
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.

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