Anticorps Monoclonal anti-Protein A

Protein A Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Staphylococcus aureus et plus (1)

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1G6A6

N° de cat : 66945-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of Recombinant protein using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

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  WB analysis of ProteinA using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

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Applications publiées

• WB: See 1 publications below

Informations sur le produit

66945-1-PBS cible Protein A dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Staphylococcus aureus

• Réactivité: Staphylococcus aureus
• Réactivité citée: levure
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Protein A
• Numéro d’acquisition GenBank: EU695225
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publications

Species	Application	Title
yeast	WB	Proc Natl Acad Sci U S A; Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stress; Authors - Siyu Fan

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