Anticorps Monoclonal anti-PRX5

• Phare
• Validé par KD/KO

PRX5 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

CloneNo.

1B8C5

N° de cat : 67599-1-Ig

Synonymes

Peroxiredoxin 5, PRDX5, 1B8C5, ACR 1, ACR1

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Galerie de données de validation

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  WB analysis using 67599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:5200 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67599-1-Ig

  WB result of PRX5 antibody (67599-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRX5 transfected HepG2 cells.

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  WB analysis using 67599-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67599-1-Ig (PRX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67599-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67599-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67599-1-Ig (PRX5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67599-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRX5 antibody (67599-1-Ig, Clone: 1B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10400
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

67599-1-Ig cible PRX5 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PRX5 Protéine recombinante Ag12262
• Nom complet: peroxiredoxin 5
• Masse moléculaire calculée: 214 aa, 22 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: BC110983
• Symbole du gène: PRX5
• Identification du gène (NCBI): 25824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxiredoxin 5 (Prx5) is a member of a family consisting of six antioxidant enzymes. Prx5 is ubiquitously expressed in various tissues including mitochondria and peroxisomes, implying that Prx5 functions as a regulator of the cellular oxidation state. Prx5 plays a critical role in protecting cells from oxidative stress by inhibiting the accumulation of reactive oxygen species and cell death. Prx5 has an intensive ROS scavenging activity because it is located in the cytosol and mitochondria. Prx5 expression was upregulated during adipogenesis and Prx5 overexpression suppressed adipogenesis by regulating cytosolic and mitochondrial ROS generation. (PMID: 29777756, PMID: 31505325, PMID: 22020876)

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
human	IHC	Cancer Med; Characterization of the Biochemical Recurrence Prediction Ability and Progression Correlation of Peroxiredoxins Family in Prostate Cancer Based on Integrating Single-Cell RNA-Seq and Bulk RNA-Seq Cohorts; Authors - Shan Tang
rat	WB,IF	Brain Res Bull; Peroxiredoxin-5 alleviates early brain injury after subarachnoid hemorrhage by reducing oxidative stress; Authors - Jinshuo Yang
human	WB,IP,IF	Int J Biol Macromol; Prebiotic stachyose inhibits PRDX5 activity and castration-resistant prostate cancer development; Authors - Rong Wang
human,mouse	IHC	Br J Cancer; Elevated serum levels of GPX4, NDUFS4, PRDX5, and TXNRD2 as predictive biomarkers for castration resistance in prostate cancer patients: an exploratory study; Authors - Rong Wang

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