Anticorps Recombinant de lapin anti-PSAP

PSAP Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250312D3

N° de cat : 86043-2-PBS

Synonymes

Prosaposin, Saposin-A, Saposin-B, Saposin-B-Val, Saposin-C

---

Galerie de données de validation

• 

  WB analysis using 86043-2-RR (same clone as 86043-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86043-2-RR (Psap antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86043-2-PBS in a different storage buffer formulation.

• 

  Sample test of MP02256-1

  The mean Psap concentration was determined to be 491.7 ng/mL in mouse serum.

• 

  IF Staining of NIH/3T3 using 86043-2-RR (same clone as 86043-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PSAP antibody (86043-2-RR, Clone: 250312D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86043-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP02256-1

  Cytometric bead array standard curve of MP02256-1, MOUSE Psap Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86043-2-PBS. Detection antibody: 86043-1-PBS. Standard: Eg3155. Range: 0.313-40 ng/mL.

---

Informations sur le produit

86043-2-PBS cible PSAP dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PSAP Protéine recombinante Eg3155
• Nom complet: prosaposin
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: NM_011179.3
• Symbole du gène: Psap
• Identification du gène (NCBI): 19156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Prosaposin (PSAP) is also named as Sulfated glycoprotein 1 (SGP-1). PSAP is a secreted neurotrophic factor and a lysosomal protein serving as the precursor of saposins (A-D), which are cofactors for hydrolases of sphingolipids (PMID: 25130661, PMID: 31220974). PSAP modulates glycosphingolipid metabolism and variants have been linked to Parkinson's disease (PD) (PMID: 37726325). As a dual-function protein, PSAP is a lysosome-associated protein that participates in a variety of cellular processes. In the lysosome, PSAP is processed to activate enzymes that degrade lipids. PSAP proteins located extracellularly are involved in cancer progression, such as proliferation and tumor death suppression signaling (PMID: 39405903). Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D (PMID: 33242172). Both human and mouse prosaposin are 65-70 kDa proteins cleaved in the lysosomes into four 10-15 kDa proteins designated saposins A, B, C and D (PMID: 8573994). Sulfated glycoprotein-1 (prosaposin) exists in 2 forms: a 65 kDa form targeted to lysosomes and a post-translationally modified to the 70 kDa form secreted extracellularly (PMID: 8593668).

{{ptg:RelatedPrimaryAntibodies}}