Anticorps Polyclonal de lapin anti-QDPR

QDPR Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (1)

N° de cat : 14908-1-AP

Synonymes

DHPR, Dihydropteridine reductase, HDHPR, PKU2, QDPR, SDR33C1

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Galerie de données de validation

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  WB analysis of mouse liver using 14908-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 14908-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14908-1-AP (QDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14908-1-AP

  IP result of anti-QDPR (IP:14908-1-AP, 4ug; Detection:14908-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14908-1-AP (QDPR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14908-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14908-1-AP (QDPR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

14908-1-AP cible QDPR dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: QDPR Protéine recombinante Ag6705
• Nom complet: quinoid dihydropteridine reductase
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC000576
• Symbole du gène: QDPR
• Identification du gène (NCBI): 5860
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dihydropteridine reductase (QDPR), also named as DHPR and HDHPR, is an essential enzyme in the hydroxylating system of the aromatic amino acids, since it catalyses the regeneration of tetrahydrobiopterin (BH4), the natural cofactor of phenylalanine, tyrosine, and tryptophan hydroxylases, from the quininoid-dihydrobiopterin produced in these coupled reactions(PMID:8326489). The QDPR protein is active as a dimer, with a subunit Mr of 26 kDa(PMID:7627180). This protein belongs to the short-chain dehydrogenases/reductases (SDR) family. Defects in QDPR are the cause of BH4-deficient hyperphenylalaninemia type C (HPABH4C)(PMID:11153907).

Publications

Species	Application	Title
mouse	WB,IF	Glia; Developmental maturation and regional heterogeneity but no sexual dimorphism of the murine CNS myelin proteome; Authors - Sophie B Siems
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
mouse	WB	Front Mol Neurosci; Broad Influence of Mutant Ataxin-3 on the Proteome of the Adult Brain, Young Neurons, and Axons Reveals Central Molecular Processes and Biomarkers in SCA3/MJD Using Knock-In Mouse Model.; Authors - Kalina Wiatr
human	WB	Genes Genomics; Subproteomic profiling from renal cortices in OLETF rats reveals mutations of multiple novel genes in diabetic nephropathy.; Authors - Zhiguo Li
human	WB	Sci Rep; Identification and validation of diagnostic biomarkers for temporal lobe epilepsy related to ferroptosis and potential therapeutic targets; Authors - Dai Shi

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Very good antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver and mouse brain

QDPR Antibody Western Blot validation (1:1000 dilution) in Mouse liver and mouse brain (Cat no:14908-1-AP)

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