Anticorps Polyclonal de lapin anti-QPRT

• Phare
• Validé par KD/KO

QPRT Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(8)

Avis (1)

N° de cat : 25174-1-AP

Synonymes

EC:2.4.2.19, Nicotinate-nucleotide pyrophosphorylase [carboxylating], QAPRTase, QPRTase

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Galerie de données de validation

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  WB analysis using 25174-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25174-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 25174-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 25174-1-AP (QPRT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 25174-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using QPRT antibody (25174-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

25174-1-AP cible QPRT dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: QPRT Protéine recombinante Ag17680
• Nom complet: quinolinate phosphoribosyltransferase
• Masse moléculaire calculée: 297 aa, 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC018910
• Symbole du gène: QPRT
• Identification du gène (NCBI): 23475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

QPRT, QAPRTase and QPRTase, is Nicotinate-nucleotide pyrophosphorylase which involved in the catabolism of quinolinic acid (QA). There're some isoforms with MW 31-33 kDa, 37 kDa and 42 kDa.

Publications

Species	Application	Title
human	IHC	Front Genet; Copy Number Variations Analysis Identifies QPRT as a Candidate Gene Associated With Susceptibility for Solitary Functioning Kidney.; Authors - Xiao Y Zhou
human		J Immunol; Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues; Authors - Teresa W-M Fan
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin
mouse	IF	Aging Cell; Deletion of enzymes for de novo NAD+ biosynthesis accelerated ovarian aging; Authors - Qingling Yang
mouse,human	WB	Nat Commun; Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas; Authors - Jianxing Yin ; KD Validated
mouse	WB,IHC,IF	Front Endocrinol (Lausanne); Unraveling the nexus of NAD+ metabolism and diabetic kidney disease: insights from murine models and human data; Authors - Sisi Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kus (Verified Customer) (12-08-2022)

Antibody works for WB in 1:500 or 1;1000 dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

QPRT Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:25174-1-AP)

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