Anticorps Polyclonal de lapin anti-QRICH1

• Phare
• Validé par KD/KO

QRICH1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30236-1-PBS

Synonymes

Transcriptional regulator QRICH1, Glutamine-rich protein 1, Glutamine rich protein 1, glutamine rich 1

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Galerie de données de validation

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  WB analysis using 30236-1-AP (same clone as 30236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 30236-1-AP (same clone as 30236-1-PBS)

  WB result of QRICH1 antibody (30236-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-QRICH1 transfected Jurkat cells. This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  WB analysis using 30236-1-AP (same clone as 30236-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 30236-1-AP (same clone as 30236-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 30236-1-AP (same clone as 30236-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using QRICH1 antibody (30236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30236-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30236-1-PBS cible QRICH1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: QRICH1 Protéine recombinante Ag31997
• Nom complet: glutamine-rich 1
• Poids moléculaire observé: 85-110 kDa
• Numéro d’acquisition GenBank: BC110855
• Symbole du gène: QRICH1
• Identification du gène (NCBI): 54870
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Glutamine-rich protein 1 (QRICH1) is a transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PMID:33384352). QRICH1 is a member of the caspase recruitment domain (CARD)-containing gene family, which encodes for proteins implicated in regulation of caspase activation in the context of apoptosis and inflammation, and in regulation of NF-κB signaling(PMID:33384352). QRICH1 can be modified by phosphorylation.

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