Anticorps Polyclonal de lapin anti-QSOX1

• Phare
• Validé par KD/KO

QSOX1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12713-1-AP

Synonymes

hQSOX, Q6, QSCN6, QSOX1, Quiescin Q6, Sulfhydryl oxidase 1

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Galerie de données de validation

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  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12713-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 12713-1-AP

  IP Result of anti-QSOX1 (IP:12713-1-AP, 4ug; Detection:12713-1-AP 1:300) with SW 1990 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SW-1990, tissu cardiaque de rat, tissu cardiaque de souris, tissu placentaire humain
• Résultats positifs en IP: cellules SW-1990
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de côlon de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

12713-1-AP cible QSOX1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: QSOX1 Protéine recombinante Ag3396
• Nom complet: quiescin Q6 sulfhydryl oxidase 1
• Masse moléculaire calculée: 747 aa, 83 kDa
• Poids moléculaire observé: 66-83kDa
• Numéro d’acquisition GenBank: BC017692
• Symbole du gène: QSOX1
• Identification du gène (NCBI): 5768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).

Publications

Species	Application	Title
human	IHC	Redox Biol; Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation.; Authors - Jialei Sun
	WB	Antioxid Redox Signal; The Emerging Role of QSOX1 in Cancer.; Authors - Douglas F Lake
human	IHC	Breast Cancer Res; High expression of QSOX1 reduces tumorogenesis, and is associated with a better outcome for breast cancer patients.; Authors - Pernodet Nicolas N
human	WB,IHC	Breast Cancer Res; Expression of quiescin sulfhydryl oxidase 1 is associated with a highly invasive phenotype and correlates with a poor prognosis in Luminal B breast cancer.; Authors - Katchman Benjamin A BA
human	IHC	Mod Pathol; QSOX1 expression is associated with aggressive tumor features and reduced survival in breast carcinomas.; Authors - Gøril Knutsvik
human	IHC	Oncotarget; Reduced QSOX1 enhances radioresistance in nasopharyngeal carcinoma.; Authors - Lei Zhou

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