Anticorps Polyclonal de lapin anti-RAB10

• Phare
• Validé par KD/KO

RAB10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(28)

Avis (1)

N° de cat : 11808-1-AP

Synonymes

Ras-related protein Rab-10, Ras related protein Rab 10, EC:3.6.5.2

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Galerie de données de validation

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  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11808-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11808-1-AP

  WB result of RAB10 antibody (11808-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB10 transfected HeLa cells.

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  WB analysis of NIH/3T3 using 11808-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11808-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 11808-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 11808-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11808-1-AP (RAB10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11808-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11808-1-AP (RAB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 11808-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using RAB10 antibody (11808-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C6, cellules HeLa, cellules MCF-7, cellules MDCK, cellules NIH/3T3, tissu cérébral de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Informations sur le produit

11808-1-AP cible RAB10 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB10 Protéine recombinante Ag2473
• Nom complet: RAB10, member RAS oncogene family
• Masse moléculaire calculée: 200 aa, 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC000896
• Symbole du gène: RAB10
• Identification du gène (NCBI): 10890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB10 belongs to the RAS superfamily of small GTPases. RAB10 localizes to a system of tubules and vesicles, and it may be involved in vesicular trafficking and neurotransmitter release. It is also associated with Glut4 translocation in adipocytes.

Publications

Species	Application	Title
rat	WB,IP,IF	Cell Res; MARCKS regulates membrane targeting of Rab10 vesicles to promote axon development.; Authors - Xiao-Hui Xu
rat, zebrafish	WB,IF	Nat Commun; Myosin Vb controls biogenesis of post-Golgi Rab10 carriers during axon development.; Authors - Liu Yang Y
mouse	IF	Proc Natl Acad Sci U S A; Ras-related protein Rab10 facilitates TLR4 signaling by promoting replenishment of TLR4 onto the plasma membrane.; Authors - Wang Di D
mouse	WB	Curr Biol; Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons; Authors - Sara C Sousa
human	WB	Int J Biol Macromol; Dihydroartemisinin requires NR1D1 mediated Rab7 ubiquitination to regulate hepatic stellate cells lipophagy in liver fibrosis; Authors - Zhengyang Bao
rat	WB	Elife; Rab10 regulates neuropeptide release by maintaining Ca2+ homeostasis and protein synthesis; Authors - Jian Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nuo (Verified Customer) (11-07-2024)

It is okay to use it to probe endogenous Rab10 but it may need a higher concentration.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse brain lysate

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