Anticorps Recombinant de lapin anti-RAB10

RAB10 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251744G12

N° de cat : 86787-1-RR

Synonymes

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

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Galerie de données de validation

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  WB analysis using 86787-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86787-1-RR (RAB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86787-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using RAB10 antibody (86787-1-RR, Clone: 251744G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86787-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86787-1-RR against Human RAB10 were performed. The affinity constant is 3.91 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules C2C12, cellules HUVEC, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF/ICC: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86787-1-RR cible RAB10 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAB10 Protéine recombinante Ag25830
• Nom complet: RAB10, member RAS oncogene family
• Masse moléculaire calculée: 200 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC000896
• Symbole du gène: RAB10
• Identification du gène (NCBI): 10890
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.

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