Anticorps Monoclonal anti-RAB11A

RAB11A Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

4A4C9

N° de cat : 67902-1-PBS

Synonymes

Rab 11, RAB11, RAB11A/B, 4A4C9, EC:3.6.5.2

---

Galerie de données de validation

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67902-1-Ig (same clone as 67902-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67902-1-Ig (same clone as 67902-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67902-1-Ig (same clone as 67902-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67902-1-Ig (same clone as 67902-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67902-1-Ig (RAB11A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67902-1-Ig (same clone as 67902-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using RAB11A antibody (67902-1-Ig, Clone: 4A4C9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67902-1-Ig (same clone as 67902-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RAB11A Monoclonal antibody (67902-1-Ig, Clone:4A4C9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67902-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67902-1-PBS cible RAB11A dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB11A Protéine recombinante Ag8879
• Nom complet: RAB11A, member RAS oncogene family
• Masse moléculaire calculée: 216 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC013348
• Symbole du gène: RAB11A
• Identification du gène (NCBI): 8766
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab11a, Rab11b and Rab25 comprise a subfamily of Rab proteins that belong to the Ras-related small GTPase protein superfamily (PMID: 8074219, PMID: 10708602). Rab11 proteins are involved in endocytosis and plasma membrane recycling in non-polarized and polarized cells ( PMID: 8638719, PMID: 8922376). Rab11a and Rab11b are ubiquitously expressed in most tissues, while Rab25 is only expressed in epithelial cells. Rab11a is known to regulate the recycling of internalized cell surface proteins and receptors from the early endosome through the trans-Golgi network (PMID: 11121436, PMID: 9600939).

{{ptg:RelatedPrimaryAntibodies}}