Anticorps Polyclonal de lapin anti-RAB11FIP2

• Phare
• Validé par KD/KO

RAB11FIP2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18136-1-PBS

Synonymes

NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2

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Galerie de données de validation

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  WB analysis using 18136-1-AP (same clone as 18136-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 18136-1-AP (same clone as 18136-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 18136-1-AP (same clone as 18136-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 18136-1-AP (same clone as 18136-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB11FIP2 antibody (18136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18136-1-AP (same clone as 18136-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 18136-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18136-1-PBS cible RAB11FIP2 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB11FIP2 Protéine recombinante Ag12736
• Nom complet: RAB11 family interacting protein 2 (class I)
• Masse moléculaire calculée: 512 aa, 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC075073
• Symbole du gène: RAB11FIP2
• Identification du gène (NCBI): 22841
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .

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