Anticorps Polyclonal de lapin anti-RAB11FIP5

• Phare
• Validé par KD/KO

RAB11FIP5 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 14594-1-AP

Synonymes

GAF1, Gaf-1, Gamma-SNAP-associated factor 1, Rab11 family-interacting protein 5, Rab11-interacting protein Rip11

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Galerie de données de validation

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293T, cellules HeLa, cellules Jurkat, tissu cardiaque de rat, tissu cardiaque de souris, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu de côlon de rat, tissu de côlon de souris, tissu rénal de rat, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

14594-1-AP cible RAB11FIP5 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB11FIP5 Protéine recombinante Ag6128
• Nom complet: RAB11 family interacting protein 5 (class I)
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC035013
• Symbole du gène: RAB11FIP5
• Identification du gène (NCBI): 26056
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He ; KD Validated
mouse,human	WB,IF	J Extracell Vesicles; Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells; Authors - Sara Busatto
	IF	J Cell Sci; Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families; Authors - Beverley Wilson

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