Anticorps Polyclonal de lapin anti-RAB12

• Phare
• Validé par KD/KO

RAB12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 18843-1-AP

Synonymes

RAB12, Ras related protein Rab 12

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Galerie de données de validation

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  WB analysis using 18843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18843-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18843-1-AP

  IP Result of anti-RAB12 (IP:18843-1-AP, 4ug; Detection:18843-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18843-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB12 antibody (18843-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu cérébral humain fœtal, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IP: See 1 publications below

Informations sur le produit

18843-1-AP cible RAB12 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB12, member RAS oncogene family
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: NM_001025300
• Symbole du gène: RAB12
• Identification du gène (NCBI): 201475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.

Publications

Species	Application	Title
human	WB	Curr Biol; Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.; Authors - C Alexander Boecker
human	WB	EMBO Rep; Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy.; Authors - Jie Xu
human	WB	Neurobiol Dis; Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment.; Authors - Jillian H Kluss
mouse	WB	J Immunol; Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.; Authors - Adi Efergan ; KD Validated
human	WB	Front Oncol; Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.; Authors - Yujie Huang ; KD Validated
human	WB	Traffic; Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.; Authors - Gustaf E Rydell ; KD Validated