Anticorps Polyclonal de lapin anti-RAB13

• Phare
• Validé par KD/KO

RAB13 Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31416-1-PBS

Synonymes

GIG4, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13, Ras-related protein Rab-13

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Galerie de données de validation

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  WB analysis using 31416-1-AP (same clone as 31416-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31416-1-AP (RAB13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 31416-1-AP (same clone as 31416-1-PBS)

  WB result of RAB13 antibody (31416-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells. This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31416-1-AP (same clone as 31416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 31416-1-AP (same clone as 31416-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 31416-1-AP (RAB13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 31416-1-AP (same clone as 31416-1-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.8 ug RAB13 Polyclonal antibody (31416-1-AP) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 31416-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31416-1-PBS cible RAB13 dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB13 Protéine recombinante Ag34898
• Nom complet: RAB13, member RAS oncogene family
• Masse moléculaire calculée: 203 aa, 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC000799
• Symbole du gène: RAB13
• Identification du gène (NCBI): 5872
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).

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