Anticorps Polyclonal de lapin anti-RAB17

• Phare
• Validé par KD/KO

RAB17 Polyclonal Antibody for IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 17501-1-AP

Synonymes

Ras-related protein Rab-17, Ras related protein Rab 17

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Galerie de données de validation

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  IHC staining of human pancreas using 17501-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17501-1-AP (RAB17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 17501-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17501-1-AP (RAB17 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of WIF-B cells using 17501-1-AP

  IF result of RAB17 antibody (17501-1-AP) with WIF-B cells overexpressed myc-tagged RAB17 by A.C. Striz and Dr. P.L. Tuma.

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Applications testées

• Résultats positifs en IHC: tissu pancréatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules WIF-B

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:25-1:50

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

17501-1-AP cible RAB17 dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB17 Protéine recombinante Ag11688
• Nom complet: RAB17, member RAS oncogene family
• Masse moléculaire calculée: 212 aa, 23 kDa
• Numéro d’acquisition GenBank: BC050426
• Symbole du gène: RAB17
• Identification du gène (NCBI): 64284
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells . Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.

Publications

Species	Application	Title
human	WB,IF	Mol Cancer; Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer.; Authors - Shaocong Wu ; KD Validated
human	WB,IHC,IF,CoIP	Cell Death Dis; RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis; Authors - Xing Zhou ; KD Validated
human	IHC	Int J Mol Sci; The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK; Authors - Vivien Li
human	IF	Cell Death Dis; Rab17 mediates differential antigen sorting following efferocytosis and phagocytosis.; Authors - Charles Yin
	WB	J Biol Chem; The GTP-bound and Sumoylated Form of the rab17 Small Molecular Weight GTPase Selectively Binds Syntaxin 2 in Polarized Hepatic WIF-B Cells.; Authors - Anneliese C Striz
	WB	Mol Biol Cell; Rab17 Regulates apical delivery of hepatic transcytotic vesicles.; Authors - Anneliese C Striz

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