Anticorps Recombinant de lapin anti-RAB21

RAB21 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

242117C4

N° de cat : 84776-2-RR

Synonymes

Ras related protein Rab 21, KIAA0118, 242117C4

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Galerie de données de validation

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  WB analysis using 84776-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84776-2-RR (RAB21 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 84776-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84776-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 84776-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 84776-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 84776-2-RR (RAB21 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 84776-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB21 antibody (84776-2-RR, Clone: 242117C4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84776-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84776-2-RR against Human RAB21 were performed. The affinity constant is 0.388 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules LNCaP, cellules MCF-7, cellules MDA-MB-231
• Résultats positifs en IHC: tissu de côlon humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84776-2-RR cible RAB21 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAB21 Protéine recombinante Ag35291
• Nom complet: RAB21, member RAS oncogene family
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_014999
• Symbole du gène: RAB21
• Identification du gène (NCBI): 23011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Small GTPase Rab21 is a ubiquitously expressed and has recently been shown to associate with the α tails of several integrins via the shared conserved membrane proximal sequence, thus regulating cell adhesion and migration via controlling the endo/exocytic trafficking of most integrin heterodimers (PMID: 18804435). Rab21 is required for the pruning of the immature neurites during the multipolar-to-bipolar transition (PMID: 36683567). Knock down of Rab21 impairs integrin-mediated cell adhesion and motility, whereas its overexpression stimulates cell migration and cancer cell adhesion to collagen and human bone (PMID: 16754960).

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