Anticorps Polyclonal de lapin anti-RAB25

• Phare
• Validé par KD/KO

RAB25 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 13189-1-AP

Synonymes

CATX 8, CATX8, CATX-8, Ras related protein Rab 25, Ras-related protein Rab-25

---

Galerie de données de validation

• 

  WB analysis of HT-29 using 13189-1-AP

  HT-29 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 13189-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 13189-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 13189-1-AP (RAB25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 13189-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13189-1-AP (RAB25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HT-29, cellules Caco-2, cellules T-47D
• Résultats positifs en IHC: tissu de carcinome à cellules rénales humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

13189-1-AP cible RAB25 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB25 Protéine recombinante Ag3864
• Nom complet: RAB25, member RAS oncogene family
• Masse moléculaire calculée: 213 aa, 23 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC033322
• Symbole du gène: RAB25
• Identification du gène (NCBI): 57111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	FEBS J; The protease ADAMTS5 controls ovarian cancer cell invasion, downstream of Rab25; Authors - Shengnan Yuan ; KD Validated
human	IHC	Transl Cancer Res; Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma; Authors - Tianyu Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Elena (Verified Customer) (05-13-2022)

The antibody detected a single band at the expected molecular weight, under both reducing and non-reducing conditions

• Applications: Western Blot
• Primary Antibody Dilution: 1:600
• Cell Tissue Type: Ovarian cancer cells

{{ptg:RelatedPrimaryAntibodies}}