Anticorps Monoclonal anti-RAB27A

• Phare
• Validé par KD/KO

RAB27A Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C4B8

N° de cat : 66058-1-PBS

Synonymes

1C4B8, EC:3.6.5.2, GTP binding protein Ram, GTP-binding protein Ram, Rab 27

---

Galerie de données de validation

• 

  WB analysis using 66058-1-Ig (same clone as 66058-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K562 using 66058-1-Ig (same clone as 66058-1-PBS)

  WB result of RAB27A antibody (66058-1-Ig, 1:5000) with si-Control and si-RAB27A transfected K562 cells. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66058-1-Ig (same clone as 66058-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66058-1-Ig (RAB27A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66058-1-Ig (RAB27A antibody) at dilution of 1:10000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skeletal muscle using 66058-1-Ig (same clone as 66058-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 66058-1-Ig(RAB27A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66058-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66058-1-PBS cible RAB27A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB27A Protéine recombinante Ag12382
• Nom complet: RAB27A, member RAS oncogene family
• Masse moléculaire calculée: 221 aa, 25 kDa
• Poids moléculaire observé: 27 kDa
• Numéro d’acquisition GenBank: BC107680
• Symbole du gène: RAB27A
• Identification du gène (NCBI): 5873
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab27A is a member of a large family of Ras-related small GTPases. The protein is membrane-bound and may be involved in protein transport and small GTPase mediated signal transduction. Recent studies suggest that Rab27A is associated with melanosomes in pigmented cells and regulates melanosome transport via its interaction with actin-based cellular motors such as myosin Va and myosin VIIa. RAB27A is also required for regulated secretion in cytotoxic T lymphocytes. Rab27A is implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal.

{{ptg:RelatedPrimaryAntibodies}}