Anticorps Monoclonal anti-RAB27B

• Phare
• Validé par KD/KO

RAB27B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B9G9

N° de cat : 66944-1-PBS

Synonymes

C25KG, Ras-related protein Rab-27B, Ras related protein Rab 27B, EC:3.6.5.2, 2B9G9

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Galerie de données de validation

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  WB analysis using 66944-1-Ig (same clone as 66944-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 66944-1-Ig (same clone as 66944-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66944-1-Ig (RAB27B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66944-1-Ig (same clone as 66944-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66944-1-Ig (RAB27B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 66944-1-Ig (same clone as 66944-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RAB27B antibody (66944-1-Ig, Clone: 2B9G9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 66944-1-Ig (same clone as 66944-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human RAB27B (66944-1-Ig, Clone:2B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66944-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66944-1-PBS cible RAB27B dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB27B Protéine recombinante Ag18756
• Nom complet: RAB27B, member RAS oncogene family
• Masse moléculaire calculée: 218 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC027474
• Symbole du gène: RAB27B
• Identification du gène (NCBI): 5874
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Rab27 GTPase subfamily consists of two closely related homologs, Rab27a and Rab27b. Northern blot analysis revealed that Rab27b is expressed significantly only in testis, in which the predominant transcript was 1.4 kb in size. An 8-kb mRNA of unknown origin was detected in many tissues. Rab27b is associated with tubulovesicle membranes in the parietal cell and Rab27b may play a role in stimulation-associated membrane recruitment and gastric acid secretion. Rab27b is recently reported as a marker for breast cancer progression. It regulates invasive growth and metastasis in ER-positive breast cancer cell lines, and increased expression is associated with poor prognosis in humans.

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