Anticorps Polyclonal de lapin anti-RAB31

• Phare
• Validé par KD/KO

RAB31 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 15485-1-AP

Synonymes

RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

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Galerie de données de validation

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  WB analysis using 15485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15485-1-AP

  WB result of RAB31 antibody (15485-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB31 transfected HeLa cells.

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  WB analysis using 15485-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15485-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 15485-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 15485-1-AP

  IP result of anti-RAB31 (IP:15485-1-AP, 4ug; Detection:15485-1-AP 1:1000) with K-562 cells lysate 1640 ug.

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  IHC staining of human liver cancer using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15485-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15485-1-AP (RAB31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562, tissu placentaire humain
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

15485-1-AP cible RAB31 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB31 Protéine recombinante Ag7833
• Nom complet: RAB31, member RAS oncogene family
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC001148
• Symbole du gène: RAB31
• Identification du gène (NCBI): 11031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes.

Publications

Species	Application	Title
human	WB	J Transl Med; From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses; Authors - Tang Biao
human	WB,IF	J Extracell Biol; RAB31 drives extracellular vesicle fusion and cancer-associated fibroblast formation leading to oxaliplatin resistance in colorectal cancer; Authors - Yu-Chan Chang

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