Anticorps Polyclonal de lapin anti-RAB31-specific

• Phare
• Validé par KD/KO

RAB31-specific Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 16182-1-AP

Synonymes

RAB31, RAB22B, Ras related protein Rab 31, Ras-related protein Rab-22B, Ras-related protein Rab-31

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Galerie de données de validation

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  WB analysis using 16182-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16182-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16182-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16182-1-AP (RAB31-specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16182-1-AP (RAB31-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16182-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16182-1-AP (RAB31-specific Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu cérébral humain
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

16182-1-AP cible RAB31-specific dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: RAB31, member RAS oncogene family
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_006868
• Symbole du gène: RAB31
• Identification du gène (NCBI): 11031
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB 31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes. This antibody can bind RAB31 specifically, and not react RAB22a.

Publications

Species	Application	Title
human	WB,IF	Cell Res; RAB31 marks and controls an ESCRT-independent exosome pathway.; Authors - Denghui Wei ; KD Validated
human	WB	Int J Biol Sci; Rab31 promotes the invasion and metastasis of cervical cancer cells by inhibiting MAPK6 degradation.; Authors - Yujie Huang ; KD Validated
human	WB,IHC,CoIP	Front Oncol; RAB31 Targeted by MiR-30c-2-3p Regulates the GLI1 Signaling Pathway, Affecting Gastric Cancer Cell Proliferation and Apoptosis.; Authors - Chao-Tao Tang ; KD Validated
human	WB	PLoS One; Human liver cell trafficking mutants: characterization and whole exome sequencing.; Authors - Fei Yuan
mouse,human	WB,IHC,IF	Int J Biochem Cell Biol; Rab31 promotes activation of hepatic stellate cells by accelerating TGF-β receptor II complex endocytosis.; Authors - Chan Qiu ; KD Validated
human	WB,IHC	Life Sci; Upregulation of Rab31 is associated with poor prognosis and promotes colorectal carcinoma proliferation via the mTOR/p70S6K/Cyclin D1 signalling pathway.; Authors - Li Yang ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Edward (Verified Customer) (11-15-2022)

Showed up reasonably well using 12 ug protein lysate with fluorescent secondary

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: K562, KCL22 and THP-1

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