Anticorps Polyclonal de lapin anti-RAB32

• Phare
• Validé par KD/KO

RAB32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(3)

Avis (1)

N° de cat : 10999-1-AP

Synonymes

Ras-related protein Rab-32, Ras related protein Rab 32

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Galerie de données de validation

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  WB analysis using 10999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10999-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 10999-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10999-1-AP (RAB32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 10999-1-AP

  IP result of anti-RAB32 (IP:10999-1-AP, 4ug; Detection:10999-1-AP 1:500) with HEK-293T cells lysate 1560 ug.

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  IHC staining of human colon using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 10999-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10999-1-AP (RAB32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 10999-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (10999-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules HepG2, tissu de côlon humain
• Résultats positifs en IP: cellules HEK-293T,
• Résultats positifs en IHC: tissu de côlon humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

10999-1-AP cible RAB32 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB32 Protéine recombinante Ag1450
• Nom complet: RAB32, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC015061
• Symbole du gène: RAB32
• Identification du gène (NCBI): 10981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Cell Death Dis; Rab32 promotes glioblastoma migration and invasion via regulation of ERK/Drp1-mediated mitochondrial fission; Authors - Pin Chen ; KD Validated
mouse	WB,IF	mSphere; Burkholderia pseudomallei BopE suppresses the Rab32-dependent defense pathway to promote its intracellular replication and virulence; Authors - Chenglong Rao ; KD Validated
human,mouse	WB,CoIP	J Cell Biol; LYSMD proteins promote activation of Rab32-family GTPases for lysosome-related organelle biogenesis; Authors - Jinglin Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

deng (Verified Customer) (01-31-2025)

The antibody detects human endogenous Rab32.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 dilution for WB, and 1: 250 dilution for IF
• Cell Tissue Type: Mouse brain injected with human AAV-Rab32, human RPE1 cells

RAB32 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution for WB, and 1: 250 dilution for IF dilution) in Mouse brain injected with human AAV-Rab32, human RPE1 cells (Cat no:10999-1-AP)

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