Anticorps Recombinant de lapin anti-RAB32

RAB32 Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230315B10

N° de cat : 82960-5-PBS

Synonymes

Ras-related protein Rab-32, Ras related protein Rab 32, 230315B10

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Galerie de données de validation

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  IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82960-5-RR (same clone as 82960-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82960-5-RR (RAB32 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  IF Staining of U-251 using 82960-5-RR (same clone as 82960-5-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using RAB32 antibody (82960-5-RR, Clone: 230315B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82960-5-RR (same clone as 82960-5-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RAB32 Recombinant antibody (82960-5-RR, Clone:230315B10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82960-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82960-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82960-5-RR against Human RAB32 were performed. The affinity constant is 0.499 nM.

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Informations sur le produit

82960-5-PBS cible RAB32 dans les applications de IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAB32 Protéine recombinante Ag1450
• Nom complet: RAB32, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Numéro d’acquisition GenBank: BC015061
• Symbole du gène: RAB32
• Identification du gène (NCBI): 10981
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab32 belongs to the Ras-like small GTPase superfamily, which regulate membrane dynamics, including membrane fusion/fission, exocytosis, and cytoskeletal trafficking. Rab32 anchors the type II regulatory subunit of protein kinase A to the mitochondrion and aids in mitochondrial fission. TRab32 also appears to be involved in autophagy and melanosome secretion.

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