Anticorps Polyclonal de lapin anti-RAB33B

RAB33B Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 27349-1-PBS

Synonymes

Ras-related protein Rab-33B, Ras related protein Rab 33B

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Galerie de données de validation

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  WB analysis of HeLa using 27349-1-AP (same clone as 27349-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  WB analysis using 27349-1-AP (same clone as 27349-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 27349-1-AP (same clone as 27349-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 27349-1-AP (same clone as 27349-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27349-1-AP (RAB33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach using 27349-1-AP (same clone as 27349-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27349-1-AP (RAB33B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27349-1-PBS in a different storage buffer formulation.

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Informations sur le produit

27349-1-PBS cible RAB33B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB33B Protéine recombinante Ag24181
• Nom complet: RAB33B, member RAS oncogene family
• Masse moléculaire calculée: 229 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC036064
• Symbole du gène: RAB33B
• Identification du gène (NCBI): 83452
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAB33B is a small GTP-binding protein of the Rab GTPase family, whose members function in vesicle transport during protein secretion and endocytosis. Rab GTPases are active, membrane-associated proteins that recruit effector proteins in the GTP-bound state and inactive cytosolic proteins when in a GDP-bound state. RAB33B is ubiquitously expressed and has been implicated in Golgi to endoplasmic reticulum cycling of Golgi enzymes. In addition, RAB33B regulates Golgi homeostasis and coordinates intra-Golgi retrograde trafficking.

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