Anticorps Polyclonal de lapin anti-RAB3A

• Phare
• Validé par KD/KO

RAB3A Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 15029-1-AP

Synonymes

RAB3A, Ras related protein Rab 3A

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Galerie de données de validation

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15029-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15029-1-AP (RAB3A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 15029-1-AP

  IP result of anti-RAB3A(IP:15029-1-AP, 4ug; Detection:15029-1-AP 1:1000) with mouse brain tissue lysate 1920 ug.

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 15029-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15029-1-AP (RAB3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules SH-SY5Y, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

15029-1-AP cible RAB3A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3A Protéine recombinante Ag1903
• Nom complet: RAB3A, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC011782
• Symbole du gène: RAB3A
• Identification du gène (NCBI): 5864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3A is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.

Publications

Species	Application	Title
human	WB,IHC,IF	Mol Oncol; Overexpression of synaptic vesicle protein Rab GTPase 3C promotes vesicular exocytosis and drug resistance in colorectal cancer cells; Authors - Yu-Chan Chang
human	IHC	Mol Cancer; Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.; Authors - Yu-Chan Chang
human	WB,IHC	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu ; KD Validated
mouse	WB	FASEB J; Role of RanBP9 on amyloidogenic processing of APP and synaptic protein levels in the mouse brain.; Authors - Lakshmana Madepalli K MK
mouse	WB	PLoS One; RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain.; Authors - Hongjie Wang
human	WB	Biochem Pharmacol; Zoledronate modulates intracellular vesicle trafficking in mast cells via disturbing the interaction of myosinVa/Rab3a and sytaxin4/VAMP7.; Authors - Shuang Liu