Anticorps Monoclonal anti-RAB3A

RAB3A Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B12E9

N° de cat : 68052-1-PBS

Synonymes

Ras-related protein Rab-3A, Ras related protein Rab 3A, 1B12E9

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Galerie de données de validation

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  WB analysis using 68052-1-Ig (same clone as 68052-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68052-1-Ig (RAB3A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 68052-1-Ig (same clone as 68052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68052-1-Ig (same clone as 68052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68052-1-Ig (same clone as 68052-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68052-1-Ig (RAB3A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68052-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68052-1-PBS cible RAB3A dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB3A Protéine recombinante Ag8148
• Nom complet: RAB3A, member RAS oncogene family
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC011782
• Symbole du gène: RAB3A
• Identification du gène (NCBI): 5864
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab3A is a small G-protein of the Rab family, that is implicated in vesicle fusion and regulated secretion, particularly in neurotransmitter release. Rab3 subfamily small G proteins (Rab3A, Rab3B, Rab3C, and Rab3D) control the regulated exocytosis in neuronal/secretory cells. Rab3A is the most abundant Rab GTPase in brain, where it is associated with synaptic vesicles. Rab3a is believed to modulate secretion efficiency by stimulating vesicle recruitment to sites of exocytosis and/ or by recruiting regulatory molecules to the docking/ fusion machinery.

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