Anticorps Polyclonal de lapin anti-RAB3C

RAB3C Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10788-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 10788-1-AP (same clone as 10788-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 10788-1-AP (same clone as 10788-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10788-1-AP (same clone as 10788-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10788-1-AP (RAB3C antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  IP result of anti-RAB3C (IP:10788-1-AP, 4ug; Detection:10788-1-AP 1:300) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 10788-1-AP (same clone as 10788-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10788-1-AP (RAB3C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10788-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10788-1-PBS cible RAB3C dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3C Protéine recombinante Ag1241
• Nom complet: RAB3C, member RAS oncogene family
• Masse moléculaire calculée: 26 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC013033
• Symbole du gène: RAB3C
• Identification du gène (NCBI): 115827
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab proteins are small molecular weight GTPases that control vesicular traffic in eukaryotic cells. A subset of Rab proteins, the Rab3 proteins (Rab3A, Rab3B, Rab3C, and Rab3D) are thought to play an important role in regulated exocytosis of vesicles. Although Rab3 proteins are highly homologous, displaying 77-85% amino acid identity with greatest variance evident within the N- and C-terminal regions, their subcellular targets and functional roles remain somewhat distinct between them. RT-PCR analysis indicated that human Rab3C was expressed in the human brain, placenta, and lung.

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