Anticorps Polyclonal de lapin anti-RAB3GAP1

• Phare
• Validé par KD/KO

RAB3GAP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (2)

N° de cat : 21663-1-AP

Synonymes

KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3 GTPase-activating protein 1, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

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Galerie de données de validation

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  WB analysis using 21663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 21663-1-AP

  WB result of RAB3GAP1 antibody (21663-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HEK-293 cells.

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  IHC staining of human testis using 21663-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21663-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB3GAP1 antibody 21663-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HL-60, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21663-1-AP cible RAB3GAP1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3GAP1 Protéine recombinante Ag16426
• Nom complet: RAB3 GTPase activating protein subunit 1 (catalytic)
• Masse moléculaire calculée: 981 aa, 111 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC146809
• Symbole du gène: RAB3GAP1
• Identification du gène (NCBI): 22930
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB3GAP1 (RAB3 GTPase-activating protein 1) is the catalytic subunit of the heterodimeric enzyme RAB3GAP (RAB3GTPase-activating protein), a key regulator of the RAB3 cycle, which controls calcium-mediated exocytosis of neurotransmitters and hormones. Mutations in RAB3GAP1 cause Warburg Micro syndrome (OMIM 600118) and Martsolf syndrome (OMIM 212720), phenotypically overlapping autosomal recessive conditions characterized by ocular and neurodevelopmental manifestations.

Publications

Species	Application	Title
monkey	WB	J Cell Biol; Rab18 and a Rab18 GEF complex are required for normal ER structure.; Authors - Andreas Gerondopoulos ; KD Validated
human	WB	EMBO J; COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.; Authors - Chunman Li
human, mouse	WB	BMC Biol; VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase.; Authors - Yorann Baron
mouse	WB	J Neurosci; Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP.; Authors - Yan Hong
rat	WB,IF	Toxicol Lett; Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons.; Authors - Tong-Yu Wang
human	WB	Open Biol; Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation.; Authors - Mark T Handley

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Detects a band at the correct MW via western blotting. An unspecific band also detected around 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

RAB3GAP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:21663-1-AP)

FH

Bing (Verified Customer) (08-04-2021)

OK to probe RAB3GAP1, but sometimes produces an unspecific band nearby. 66688-1-Ig seems better.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

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