Anticorps Polyclonal de lapin anti-RAB3GAP2

RAB3GAP2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 24599-1-AP

Synonymes

KIAA0839, p150, Rab3 GAP p150, Rab3 GAP regulatory subunit, RAB3 GAP150, RAB3GAP150, RAB3GAP2, RGAP iso

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Galerie de données de validation

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  WB analysis using 24599-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24599-1-AP (RAB3GAP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cerebellum using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cerebellum using 24599-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24599-1-AP (RAB3GAP2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

24599-1-AP cible RAB3GAP2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAB3GAP2 Protéine recombinante Ag20254
• Nom complet: RAB3 GTPase activating protein subunit 2 (non-catalytic)
• Masse moléculaire calculée: 1393 aa, 156 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC146760
• Symbole du gène: RAB3GAP2
• Identification du gène (NCBI): 25782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAB3GAP2, also known as RAB3-GAP150, belongs to the Rab3-GAP regulatory subunit family. Rab3 GAP consists of two subunits: the catalytic subunit p130 and the noncatalytic subunit p150. Rab3 GAP is ubiquitously expressed and enriched in the synaptic soluble fraction of brain, consistent with it having a key role in neurodevelopment. RAB3-GAP150 forms the Rab3 GTPase-activating complex with RAB3-GAP130,where it constitutes the regulatory subunit, whereas the latter functions as the catalytic subunit. Defects in RAB3-GAP150 are the cause of Martsolf and Warburg Micro syndrome.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

BING (Verified Customer) (08-04-2021)

A reliable Ab to probe RAB3GAP2 with the right MW of 150 kd.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse brain and postmortembrain lysates

RAB3GAP2 Antibody Western Blot validation (1:5000 dilution) in mouse brain and postmortembrain lysates (Cat no:24599-1-AP)

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