Anticorps Monoclonal anti-RAB5A

RAB5A Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B6A5

N° de cat : 66339-1-PBS

Synonymes

RAB5, 1B6A5, EC:3.6.5.2, Ras related protein Rab 5A, Ras-related protein Rab-5A

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Galerie de données de validation

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  WB analysis of HEK-293 using 66339-1-Ig (same clone as 66339-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66339-1-Ig (same clone as 66339-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 66339-1-Ig (same clone as 66339-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66339-1-Ig (RAB5A Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 66339-1-Ig (RAB5A Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB5A antibody (66339-1-Ig, Clone: 1B6A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66339-1-Ig (same clone as 66339-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66339-1-Ig(RAB5A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66339-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66339-1-PBS cible RAB5A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc

• Réactivité: Humain, porc
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RAB5A Protéine recombinante Ag16781
• Nom complet: RAB5A, member RAS oncogene family
• Masse moléculaire calculée: 215 aa, 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: BC001267
• Symbole du gène: RAB5A
• Identification du gène (NCBI): 5868
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rab5a belongs to the Rab GTPases family proteins, which play roles in endocytosis, exocytosis, and vesicular transportation. Rab family proteins were recently reported to play important roles in human cancer progression. Rab5a was reported to be overexpressed in breast and ovarian cancers and associated with proliferation and invasion.

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