Anticorps Polyclonal de lapin anti-RABEPK/p40

RABEPK/p40 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

N° de cat : 15105-1-AP

Synonymes

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK, RABEPK/p40

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Galerie de données de validation

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  WB analysis of HepG2 using 15105-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15105-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15105-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15105-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15105-1-AP (RABEPK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15105-1-AP

  IP result of anti-RABEPK/p40 (IP:15105-1-AP, 4ug; Detection:15105-1-AP 1:1000) with HeLa cells lysate 2080 ug.

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  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 15105-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15105-1-AP (RABEPK/p40 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15105-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RABEPK/p40 antibody (15105-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 15105-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human RABEPK/p40 (15105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15105-1-AP cible RABEPK/p40 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RABEPK/p40 Protéine recombinante Ag2593
• Nom complet: Rab9 effector protein with kelch motifs
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC065725
• Symbole du gène: RABEPK
• Identification du gène (NCBI): 10244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.