Anticorps Monoclonal anti-RABEPK/p40

RABEPK/p40 Monoclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

CloneNo.

1E11A3

N° de cat : 66622-1-Ig

Synonymes

40 kDa Rab9 effector protein, p40, RAB9P40, RABEPK

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Galerie de données de validation

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  WB analysis of Jurkat using 66622-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66622-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 66622-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66622-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 66622-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 66622-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 66622-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66622-1-Ig (RABEPK/p40 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HEK-293, cellules HeLa, cellules HSCT6, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

66622-1-Ig cible RABEPK/p40 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RABEPK/p40 Protéine recombinante Ag7796
• Nom complet: Rab9 effector protein with kelch motifs
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC065725
• Symbole du gène: RABEPK
• Identification du gène (NCBI): 10244
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Rab9 GTPase is required for the transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network in living cells, and in an in vitro system that reconstitutes this process. P40 is an effector of Rab9 that interacts preferentially with the active form of Rab9. p40 does not interact with Rab7 or K-Ras; it also fails to bind Rab9 when it is bound to GDI. The protein is found in cytosol, yet a significant fraction (~30%) is associated with cellular membranes. P40 is a very potent transport factor in that the pure, recombinant protein can stimulate, significantly, an in vitro transport assay that measures transport of mannose 6-phosphate receptors from endosomes to the trans-Golgi network.

Publications

Species	Application	Title
human	IHC	Exp Ther Med; Management of a rare ovarian carcinosarcoma: A case report and literature review; Authors - Jun Fu