Anticorps Polyclonal de lapin anti-Rac1

• Phare
• Validé par KD/KO

Rac1 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

78

N° de cat : 24072-1-AP

Synonymes

MIG5, RAC1, RAC1b, Ras like protein TC25, TC 25, TC25

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Galerie de données de validation

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  WB analysis using 24072-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24072-1-AP (Rac1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 24072-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 24072-1-AP (RAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24072-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24072-1-AP (Rac1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 24072-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 24072-1-AP (Rac1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 72 publications below
• IHC: See 7 publications below
• IF: See 9 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Informations sur le produit

24072-1-AP cible Rac1 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Rac1 Protéine recombinante Ag17779
• Nom complet: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: BC004247
• Symbole du gène: RAC1
• Identification du gène (NCBI): 5879
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.

Publications

Species	Application	Title
human	WB	Nat Commun; Regulation of local GTP availability controls RAC1 activity and cell invasion; Authors - Anna Bianchi-Smiraglia
rat	WB,coIP	ACS Nano; Oxidation of Reduced Graphene Oxide via Cellular Redox Signaling Modulates Actin-Mediated Neurotransmission.; Authors - Yiyuan Kang
human,mouse	IHC,WB	Cancer Res; CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder.; Authors - Xin Chen
mouse	WB	Biomaterials; Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.; Authors - Miao Zhang
mouse	WB	Biomaterials; Combination antitumor immunotherapy with VEGF and PIGF siRNA via systemic delivery of multi-functionalized nanoparticles to tumor-associated macrophages and breast cancer cells.; Authors - Yudong Song
human	WB,IF	Cell Mol Immunol; DOCK2 regulates antifungal immunity by regulating RAC GTPase activity.; Authors - Xiaojian Ma

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