Anticorps Polyclonal de lapin anti-RAC2

• Phare
• Validé par KD/KO

RAC2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 20573-1-AP

Synonymes

Small G protein, Ras-related C3 botulinum toxin substrate 2, p21-Rac2, p21 Rac2, HSPC022

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Galerie de données de validation

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  WB analysis of Jurkat using 20573-1-AP

  WB result of RAC2 antibody (20573-1-AP, 1:600) with si-control and si-RAC2 transfected Jurkat cells..

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  WB analysis of Jurkat using 20573-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 20573-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20573-1-AP (RAC2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IP experiment of MOLT-4 using 20573-1-AP

  IP result of anti-RAC2 (IP:20573-1-AP, 4ug; Detection:20573-1-AP 1:500) with MOLT-4 cells lysate 1800 ug.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HL-60
• Résultats positifs en IP: cellules MOLT-4,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

20573-1-AP cible RAC2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 21 kDa
• Numéro d’acquisition GenBank: NM_002872
• Symbole du gène: RAC2
• Identification du gène (NCBI): 5880
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAC2, also named as GX, belongs to the small GTPase superfamily and Rho family. RAC2 is a plasma membrane-associated small GTPase which cycles between an active GTP-bound and inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses, such as secretory processes, phagocytose of apoptotic cells and epithelial cell polarization. RAC2 augments the production of reactive oxygen species (ROS) by NADPH oxidase. Defects in RAC2 are the cause of neutrophil immunodeficiency syndrome. The antibody is specific to RAC2.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; NEDD4L Suppresses Proliferation and Promotes Apoptosis by Ubiquitinating RAC2 Expression and Acts as a Prognostic Biomarker in Clear Cell Renal Cell Carcinoma; Authors - Manlong Qi

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