Anticorps Monoclonal anti-RACGAP1

• Phare
• Validé par KD/KO

RACGAP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

6E10A2

N° de cat : 66056-1-PBS

Synonymes

HsCYK4, HsCYK 4, Gap1, GAP, CYK4

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Galerie de données de validation

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  WB analysis of HEK-293 using 66056-1-Ig (same clone as 66056-1-PBS)

  HEK-293, Jurkat, ROS1728, PC-12, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 66056-1-Ig (same clone as 66056-1-PBS)

  WB result of RACGAP1 antibody (66056-1-Ig, 1:3000) with si-control and si-RACGAP1 transfected HEK293 cells. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 66056-1-Ig (same clone as 66056-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66056-1-Ig (same clone as 66056-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66056-1-Ig (same clone as 66056-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66056-1-Ig (RACGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66056-1-Ig (same clone as 66056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66056-1-Ig (same clone as 66056-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66056-1-Ig (RACGAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66056-1-Ig (same clone as 66056-1-PBS)

  Immunofluorescent analysis of HeLa cells, using RACGAP1 antibody 66056-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). This data was developed using the same antibody clone with 66056-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66056-1-PBS cible RACGAP1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: RACGAP1 Protéine recombinante Ag6944
• Nom complet: Rac GTPase activating protein 1
• Masse moléculaire calculée: 632 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC032754
• Symbole du gène: RACGAP1
• Identification du gène (NCBI): 29127
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.

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