Anticorps Polyclonal de lapin anti-RACK1; GNB2L1

• Phare
• Validé par KD/KO

RACK1; GNB2L1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 27592-1-AP

Synonymes

Gnb2 rs1, GNB2L1, H12.3, HLC 7, PIG21, RACK1, RACK1; GNB2L1

---

Galerie de données de validation

• 

  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 27592-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 27592-1-AP (RACK1; GNB2L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HepG2, cellules L02, tissu hépatique de rat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de l'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

27592-1-AP cible RACK1; GNB2L1 dans les applications de WB, IHC, IF, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RACK1; GNB2L1 Protéine recombinante Ag26708
• Nom complet: guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: BC019093
• Symbole du gène: RACK1
• Identification du gène (NCBI): 10399
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. RACK1 (receptor for activated protein kinase C 1), encoded by GNB2L1 gene, is a 317 amino acid guanine nucleotide-binding protein subunit beta-2-like 1 protein which is involved in the recruitment, assembly and/or regulation of a variety of signaling molecules, it contains 7 WD-repeats and is implicated in various protein interaction activities. RACK1 is a component of the 40S ribosomal subunit involved in translational repression. Recent finding suggests that RACK1 may be a new promising diagnosis biomarker and therapeutic target for non-small-cell lung cancer (NSCLC).

Publications

Species	Application	Title
human	WB,CoIP	J Clin Invest; CAP2 promotes gastric cancer metastasis by mediating the interaction between tumor cells and tumor-associated macrophages; Authors - Guohao Zhang
human,mouse	WB	Cells; Modification of RNF183 via m6A Methylation Mediates Podocyte Dysfunction in Diabetic Nephropathy by Regulating PKM2 Ubiquitination and Degradation; Authors - Dongwei Guo
human	WB,IP,IF	Mol Med; LRP11 facilitates lipid metabolism and malignancy in hepatocellular carcinoma by stabilizing RACK1 through USP5 regulation; Authors - Litao Liang
human	WB,IF,IP	J Virol; The matrix protein of respiratory syncytial virus suppresses interferon signaling via RACK1 association; Authors - Jingjing Cao
	WB	Cancers (Basel); RACK1 Promotes Meningioma Progression by Activation of NF-κB Pathway via Preventing CSNK2B from Ubiquitination Degradation; Authors - Ali Abdi Maalim
monkey	WB	Virology; RACK1 promotes porcine reproductive and respiratory syndrome virus infection in Marc-145 cells through ERK1/2 activation; Authors - Xinxian Wang ; KD Validated

{{ptg:RelatedPrimaryAntibodies}}