Anticorps Polyclonal de lapin anti-RAD17

• Phare
• Validé par KD/KO

RAD17 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 13358-1-PBS

Synonymes

RF-C/activator 1 homolog, R24L, HRAD17

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Galerie de données de validation

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  WB analysis using 13358-1-AP (same clone as 13358-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13358-1-AP (RAD17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13358-1-AP (same clone as 13358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 13358-1-AP (same clone as 13358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13358-1-AP (same clone as 13358-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13358-1-AP (RAD17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13358-1-AP (same clone as 13358-1-PBS)

  Immunofluorescent analysis of Hela cells, using RAD17 antibody 13358-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 13358-1-AP (same clone as 13358-1-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug RAD17 Polyclonal antibody (13358-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13358-1-PBS in a different storage buffer formulation.

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Informations sur le produit

13358-1-PBS cible RAD17 dans les applications de WB, IHC, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD17 Protéine recombinante Ag4171
• Nom complet: RAD17 homolog (S. pombe)
• Masse moléculaire calculée: 681 aa, 71 kDa
• Poids moléculaire observé: 71 kDa
• Numéro d’acquisition GenBank: BC032304
• Symbole du gène: RAD17
• Identification du gène (NCBI): 5884
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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