Anticorps Polyclonal de lapin anti-RAD18

• Phare
• Validé par KD/KO

RAD18 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18333-1-PBS

Synonymes

E3 ubiquitin-protein ligase RAD18, EC:2.3.2.27, hHR18, hRAD18, RING finger protein 73

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Galerie de données de validation

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  WB analysis of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  WB analysis using 18333-1-AP (same clone as 18333-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 18333-1-AP (same clone as 18333-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18333-1-AP (same clone as 18333-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 18333-1-AP (same clone as 18333-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18333-1-AP (RAD18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 18333-1-AP (same clone as 18333-1-PBS)

  IP result of anti-RAD18 (IP:18333-1-AP, 3ug; Detection:18333-1-AP 1:300) with K-562 cells lysate 2000ug. This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 18333-1-AP (same clone as 18333-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 18333-1-AP (RAD18 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18333-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18333-1-PBS cible RAD18 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD18 Protéine recombinante Ag12944
• Nom complet: RAD18 homolog (S. cerevisiae)
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 75 kDa, 85 kDa
• Numéro d’acquisition GenBank: BC001302
• Symbole du gène: RAD18
• Identification du gène (NCBI): 56852
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAD18(E3 ubiquitin-protein ligase RAD18) is lalso named as RNF73, hHR18 and belongs to the RAD18 family. It is involved in postreplication repair of UV-damaged DNA. RAD18 protein is detected in human cells as two major bands at 75 and 85 kDa by Western blot. The bands are identified as nonubiquitinated and monoubiquitinated forms of Rad18, respectively(PMID: 15509568). Monoubiquitinated RAD18 is detected mainly in the cytoplasm, whereas nonubiquitinated RAD18 is detected predominantly in the nuclei(PMID:15509568). This antibody is specific to RAD18.

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