Anticorps Recombinant de lapin anti-RAD21

• Phare
• Validé par KD/KO

RAD21 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242172E3

N° de cat : 84920-4-RR

Synonymes

hHR21, FLJ40596, FLJ25655, Double-strand-break repair protein rad21 homolog, 64-kDa C-terminal product

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Galerie de données de validation

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  WB analysis using 84920-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84920-4-RR (RAD21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 84920-4-RR

  WB result of RAD21 antibody (84920-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD21 transfected HeLa cells.

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  IF Staining of HeLa using 84920-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD21 antibody (84920-4-RR, Clone: 242172E3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 84920-4-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RAD21 Recombinant antibody (84920-4-RR, Clone:242172E3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84920-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84920-4-RR against Human RAD21 were performed. The affinity constant is 2.23 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84920-4-RR cible RAD21 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAD21 Protéine recombinante Ag25481
• Nom complet: RAD21 homolog (S. pombe)
• Masse moléculaire calculée: 631 aa, 72 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC050381
• Symbole du gène: RAD21
• Identification du gène (NCBI): 5885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD21, also named as Nuclear matrix protein 1, is a 631 amino acid protein, which belongs to the rad21 family. The calculated molecular weight of RAD21 is 72 kDa, but the phosphorylated RAD21 is about 120 kDa. The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

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