Anticorps Recombinant de lapin anti-RAD23A

RAD23A Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250716C7

N° de cat : 86132-1-RR

Synonymes

HHR23A, HR23A

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Galerie de données de validation

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  WB analysis of Jurkat using 86132-1-RR

  Various cell lysates were subjected to SDS PAGE followed by western blot with 86132-1-RR (RAD23A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86132-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86132-1-RR against Human RAD23A were performed. The affinity constant is 2.99 nM.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules C6, cellules MCF-7, cellules SKOV-3, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86132-1-RR cible RAD23A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAD23A Protéine recombinante Ag0716
• Nom complet: RAD23 homolog A (S. cerevisiae)
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC014026
• Symbole du gène: RAD23A
• Identification du gène (NCBI): 5886
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD23A, also named as HHR23A, belongs to the RAD23 family. It is a multiubiquitin chain receptor involved in modulation of proteasomal degradation. RAD23A binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. It is proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. It is involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC. It is also involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages and is required for the association of HIV-1 vpr with the host proteasome.

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