Anticorps Polyclonal de lapin anti-RAD23B

• Phare
• Validé par KD/KO

RAD23B Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 12121-1-AP

Synonymes

HHR23B, HR23B, p58, XP-C repair-complementing complex 58 kDa protein

---

Galerie de données de validation

• 

  WB analysis of HeLa using 12121-1-AP

  WB result of RAD23B antibody (12121-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAD23B transfected HeLa cells.

• 

  WB analysis using 12121-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12121-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12121-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12121-1-AP (RAD23B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12121-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12121-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12121-1-AP (RAD23B antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12121-1-AP cible RAD23B dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD23B Protéine recombinante Ag2763
• Nom complet: RAD23 homolog B (S. cerevisiae)
• Masse moléculaire calculée: 409 aa, 43 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC020973
• Symbole du gène: RAD23B
• Identification du gène (NCBI): 5887
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD23B, also named as p58, hHR23B and HR23B, belongs to the RAD23 family. It plays a central role both in proteosomal degradation of misfolded proteins and DNA repair. RAD23B is a central component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. Involved in DNA excision repair by stabilizing XPC protein. It may play a part in DNA damage recognition and/or in altering chromatin structure to allow access by damage-processing enzymes. RAD23B displays aberrant mobility in SDS-polyacrylamide gels, as it is typically detected as a 58- to 60-kDa protein despite a predicted size of 42 kDa (PMID: 8692695, 12052895).

Publications

Species	Application	Title
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human	WB,IHC	Int J Biol Sci; Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.; Authors - Jianjiang Wang
human	WB	Front Oncol; PMID: 31249811; Authors - Ma-Sha Huang
rat	WB	PLoS One; Proteins with Intrinsically Disordered Domains Are Preferentially Recruited to Polyglutamine Aggregates.; Authors - Maggie P Wear
human	WB	J Cancer; PMID: 31772670; Authors - Li-Ming Tan

{{ptg:RelatedPrimaryAntibodies}}