Anticorps Polyclonal de lapin anti-RAD50

RAD50 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29390-1-PBS

Synonymes

hRad50, RAD50 homolog (S. cerevisiae)

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Galerie de données de validation

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  WB analysis using 29390-1-AP (same clone as 29390-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29390-1-AP (RAD50 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 29390-1-AP (same clone as 29390-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29390-1-AP (RAD50 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using RAD50 antibody (29390-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29390-1-AP (same clone as 29390-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RAD50 antibody (29390-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 29390-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29390-1-PBS cible RAD50 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: RAD50 Protéine recombinante Ag29949
• Nom complet: RAD50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 154 kDa
• Numéro d’acquisition GenBank: NM_005732
• Symbole du gène: RAD50
• Identification du gène (NCBI): 10111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

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