Anticorps Recombinant de lapin anti-RAD50

RAD50 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242541A5

N° de cat : 84969-4-PBS

Synonymes

242541A5, hRad50, RAD50 homolog (S. cerevisiae)

---

Galerie de données de validation

• 

  WB analysis using 84969-4-RR (same clone as 84969-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84969-4-RR (RAD50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 84969-4-RR (same clone as 84969-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84969-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84969-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84969-4-RR against Human RAD50 were performed. The affinity constant is 6.79 nM.

---

Informations sur le produit

84969-4-PBS cible RAD50 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAD50 Protéine recombinante Ag29949
• Nom complet: RAD50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 154 kDa
• Numéro d’acquisition GenBank: NM_005732
• Symbole du gène: RAD50
• Identification du gène (NCBI): 10111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

{{ptg:RelatedPrimaryAntibodies}}