Anticorps Recombinant de lapin anti-RAD50

RAD50 Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242541A5

N° de cat : 84969-4-RR

Synonymes

242541A5, hRad50, RAD50 homolog (S. cerevisiae)

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Galerie de données de validation

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  WB analysis using 84969-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84969-4-RR (RAD50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 84969-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 84969-4-RR (RAD50 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84969-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84969-4-RR against Human RAD50 were performed. The affinity constant is 6.79 nM.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules C6, cellules Jurkat, cellules MCF-7, cellules MOLT-4
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu d'estomac de rat, tissu d'estomac de souris, tissu pulmonaire de rat, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84969-4-RR cible RAD50 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: RAD50 Protéine recombinante Ag29949
• Nom complet: RAD50 homolog (S. cerevisiae)
• Masse moléculaire calculée: 154 kDa
• Poids moléculaire observé: 154 kDa
• Numéro d’acquisition GenBank: NM_005732
• Symbole du gène: RAD50
• Identification du gène (NCBI): 10111
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RAD50 belongs to the ABC-ATPase protein family that concurs in forming the Nucleotide Binding Domain (NBD). ATP binding to Rad50 induces a conformational change that dramatically increases the Rad50 NBD affinity for itself, allowing for its dimerization (PMID: 37569756). The Mre11-Rad50 (MR) complex has key functions in the detection, signaling and repair of DNA breaks (PMID: 35501303).

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